High Precision and Sensitivity CRP PTX1 Porcine ELISA Assay Kit
Highly Sensitive With Oem Service
Standard Curve Range: 0.2ng/ml - 70ng/ml
Size: 96 wells
* This product is for research use only, not for use in diagnosis
procedures. It’s highly recommend to read this instruction entirely
Serum Allow serum to clot for 10-20 minutes at room temperature.
Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant.
Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C
within 30 minutes of collection.
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for
approximately 20 minutes. When collecting pleuroperitoneal fluid
and cerebrospinal fluid, please follow the procedures
Cell Culture Supernatant Collect by sterile tubes when examining secrete components.
Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect
the supernatants carefully. When examining the components within
the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the
cell concentration of approximately 1 million/ml. Damage cells
through repeated freeze-thaw cycles to let out the inside
components. Centrifuge at 2000-3000 RPM for approximately 20
Tissue and other body fluids Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly
and weigh before homogenization. Mince tissues and homogenize them
in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or
freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20
- Sample concentrations should be predicted before being used in the
assay. If the sample concentration is not within the range of the
standard curve, users must contact us to determine the optimal sample for their particular experiments.
- Samples to be used within 5 days should be stored at 2-8°C. Samples
should be aliquoted or must be stored at -20°C within 1 month or
-80°C within 6 months. Avoid repeated freeze thaw cycles.
- Samples should be brought to room temperature before starting the
- Centrifuge to collect sample before use.
- Samples containing NaN3 can’t be tested as it inhibits the activity
of Horse Radish Peroxidase (HRP).
- Collect the supernatants carefully. When sediments occurred during
storage, centrifugation should be performed again.
- Hemolysis can greatly impact the validity of test results. Take
care to minimize hemolysis.
*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit,
the sample matrix interference may falsely depress the specificity
and accuracy of the assay.
This elisa test kit is an Enzyme-Linked Immunosorbent Assay
(ELISA). The plate has been pre-coated with Porcine CRP/PTX1
antibody. CRP/PTX1 present in the sample is added and binds to
antibodies coated on the wells. And then biotinylated Porcine
CRP/PTX1 Antibody is added and binds to CRP/PTX1 in the sample.
Then Streptavidin-HRP is added and binds to the Biotinylated
CRP/PTX1 antibody. After incubation unbound Streptavidin-HRP is
washed away during a washing step. Substrate solution is then added
and color develops in proportion to the amount of Porcine CRP/PTX1.
The reaction is terminated by addition of acidic stop solution and
absorbance is measured at 450 nm.
|Standard Solution (80ng/ml)||0.5ml x1|
|Pre-coated ELISA Plate||12 * 8 well strips x1|
|Standard Diluent||3ml x1|
|Stop Solution||6ml x1|
|Substrate Solution A||6ml x1|
|Substrate Solution B||6ml x1|
|Wash Buffer Concentrate (25x)||20ml x1|
|Biotinylated Porcine CRP/PTX1 Antibody||1ml x1|
|Plate Sealer||2 pics|
|Zipper bag||1 pic|
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (80ng/ml) with 120μl of
standard diluent to generate a 40ng/ml standard stock solution.
Allow the standard to sit for 15 mins with gentle agitation prior
to making dilutions. Prepare duplicate standard points by serially
diluting the standard stock solution (40ng/ml) 1:2 with standard
diluent to produce 20ng/ml, 10ng/ml, 5ng/ml and 2.5ng/ml solutions.
Standard diluent serves as the zero standard(0 ng/ml). Any
remaining solution should be frozen at -20°C and used within one
month. Dilution of standard solutions suggested are as follows:
|40ng/ml||Standard No.5||120μl Original Standard + 120μl Standard Diluent|
|20ng/ml||Standard No.4||120μl Standard No.5 + 120μl Standard Diluent|
|10ng/ml||Standard No.3||120μl Standard No.4 + 120μl Standard Diluent|
|5ng/ml||Standard No.2||120μl Standard No.3 + 120μl Standard Diluent|
|2.5ng/ml||Standard No.1||120μl Standard No.2 + 120μl Standard Diluent|
|Standard Concentration||Standard No.5||Standard No.4||Standard No.3||Standard No.2||Standard No.1|
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or
distilled water to yield 500 ml of 1x Wash Buffer. If crystals have
formed in the concentrate, mix gently until the crystals have
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.