Sign In | Join Free | My isp.org.cn
China Shanghai Korain Biotech Co., Ltd logo
Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co Ltd
Verified Supplier

3 Years

Home > ELISA Test Kit >

Customized 1 96 Wells Size HAT1 ELISA Test Kit Human Histone Acetyltransferase

Shanghai Korain Biotech Co., Ltd
Trust Seal
Verified Supplier
Credit Check
Supplier Assessment

Customized 1 96 Wells Size HAT1 ELISA Test Kit Human Histone Acetyltransferase

Brand Name : BT Lab

Model Number : Cat.No E6942Hu

Certification : CE, ISO9001:2005, MSDS

Place of Origin : Shanghai, China

MOQ : Negotiation

Price : Negotiation

Supply Ability : Western Union, T/T

Delivery Time : 1-3 business days, bulk order within one week

Packaging Details : Wrapped with ice pack and styrofoam package

Product name : HAT1 ELISA Test Kit

Cat.No : E6942Hu

OEM : Acceptable

Icubate time : About 75 mins

Application : Human Histone , laboratory

Known as : HAT1

Contact Now

High Sensitive Human Histone Acetyltransferase 1 ELISA Kit Customized HAT1 ELISA Test Kit


Cat.No E6942Hu

Standard Curve Range: 0.05ng/ml - 30ng/ml

Sensitivity: 0.028ng/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.


Precautions

  • Prior to use, the sandwich elisa kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The sandwich elisa kit should not be used beyond the expiration date.

Reagent Provided

ComponentsQuantity
Standard Solution (32ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (25x)20ml x1
Biotinylated human HAT1 Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


Tissue and other body fluids Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.


Reagent Preparation


All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (32ng/ml) with 120μl of standard diluent to generate a 16ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (16ng/ml) 1:2 with standard diluent to produce 8ng/ml, 4ng/ml, 2ng/ml and 1ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


16ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
8ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
4ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
2ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
1ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
32ng/ml16ng/ml8ng/ml4ng/ml2ng/ml1ng/ml

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.


Product Tags:

elisa reagent kit

      

elisa kit

      
Cheap Customized 1 96 Wells Size HAT1 ELISA Test Kit Human Histone Acetyltransferase wholesale

Customized 1 96 Wells Size HAT1 ELISA Test Kit Human Histone Acetyltransferase Images

Inquiry Cart 0
Send your message to this supplier
 
*From:
*To: Shanghai Korain Biotech Co., Ltd
*Subject:
*Message:
Characters Remaining: (0/3000)